Breeding for resilience: enhancing multi-stress tolerance in crops
HORIZON Research and Innovation Actions
Basic Information
- Identifier
- HORIZON-CL6-2025-01-BIODIV-02-two-stage
- Programme
- Cluster 6 Call 01 - two stage
- Programme Period
- 2021 - 2027
- Status
- Closed (31094503)
- Opening Date
- May 6, 2025
- Deadline
- September 4, 2025
- Deadline Model
- two-stage
- Budget
- €10,000,000
- Min Grant Amount
- €5,000,000
- Max Grant Amount
- €5,000,000
- Expected Number of Grants
- 2
- Keywords
- HORIZON-CL6-2025-01-BIODIV-02-two-stageHORIZON-CL6-2025-01-two-stageAgriculture related to crop production, soil biology and cultivation, applied plant biologyPlant biologyPlant breedingPlant geneticsPlant physiology
Description
In line with the objectives of the European Green Deal, the common agricultural policy, the EU climate policy and the EU biodiversity strategy for 2030, a successful proposal will contribute to the expected impact of this destination by supporting the adaptation of agricultural production to the effects of climate change, increasing biodiversity in agroecosystems, and promoting low-input practices, thereby enhancing the resilience of agricultural systems and safeguarding food security.
Projects are expected to contribute to all of the following expected outcomes:
- deeper knowledge and characterisation of relevant traits for tolerance and resistance to multiple stresses, whether occurring simultaneously or sequentially, are more accessible to researchers and breeders;
- the identification of local varieties with high plasticity to cope with multi-stress conditions is enhanced, along with the development of agro-ecological practices that improve stress tolerance while supporting biodiversity-friendly cropping systems;
- the capacities to evaluate the effects of multiple stresses in crops by researchers and breeders are strengthened;
- information and recommendations on variety performance and practices to cope with multi-stress are available to advisors and farmers.
Crop production faces significant challenges due to climate change and the need to adopt low-input practices, including efficient water use, to reduce the environmental impact while ensuring food security. Issues such as salinity, extreme weather conditions like droughts, waterlogging, high temperatures, and emerging patterns of pests and diseases severely impact crops, resulting in reduced productivity and yield losses. Crop responses to multiple stresses differ from their responses to single stresses. Therefore, attention should be given to enhancing crop tolerance to combinations of multiple abiotic and biotic stresses, thus better reflecting real-life agricultural conditions.
To address these challenges, it is crucial to evaluate local crop varieties, which are often better adapted to specific environmental conditions and stresses. Identifying local varieties with high plasticity enhances crop resilience and agro-biodiversity. Developing agro-ecological practices to improve stress tolerance will further support these efforts, promoting low-input practices and enhancing the overall adaptability of agricultural systems. Additionally, broad-spectrum strategies for improving stress tolerance in crops should be developed. Smart and future-proof breeding programmes need to systematically consider characteristics that enhance crop resilience and adaptation to these demands.
Proposals should:
- provide insight into the range of mechanisms and traits that underpin crop responses to multiple stresses, whether occurring simultaneously or sequentially, guiding the development of varieties and a crop system better equipped to withstand abiotic and biotic stresses, including reduced agricultural inputs;
- increase understanding of the causality between abiotic and biotic stress factors and propose strategies to improve multi-stress tolerance;
- integrate advanced technologies to assist in evaluating GxExM (Genotype x Environment x Management) interactions in the context of multi-stress, combining multiple "omics" data sources, high-throughput phenotyping, computational modelling and artificial intelligence, to evaluate at different levels (e.g. greenhouses, experimental fields, production fields). This integration should assist breeders in developing local varieties optimised for sustainability and climate change adaptation;
- develop location-specific breeding strategies and agroecological practices, incorporating models and artificial intelligence approaches for prediction of cropping systems output, under multiple stress conditions considering climate change scenarios and climate analogues. These strategies should promote agrobiodiversity, soil health, and ecosystem services;
- deliver robust methodologies for benchmarking and communicating the performance of crop varieties when they are challenged by multiple stresses.
Proposals should provide a clear explanation and justification for the selected crop(s) in alignment with the proposal’s objectives and the topic’s expected outcomes, considering as well that activities should be carried out in a range of agronomically relevant pedo-climatic conditions. All farming systems and approaches are in scope. If proposals address organic farming, particular attention should be given to aspects related to organic varieties and organic heterogeneous materials.
Proposals may provide financial support to third parties (FSTP) to, for instance, develop, test and demonstrate tools to evaluate GxExM interactions in the context of multi-stress. A maximum of 20% of the EU funding should be allocated to this purpose. Consortia need to define the selection process of organisations, for which financial support may be granted.
Proposals should ensure coherence and complementarities with ongoing relevant Horizon Europe projects, including the agroecology partnership, and capitalise on existing relevant research findings and tools, included those developed under previous research projects. Collaboration with European research infrastructures such as AnaEE-ERIC, EMPHASIS or other relevant research infrastructures[1] is encouraged.
[1] The catalogue of European Strategy Forum on Research Infrastructures (ESFRI) research infrastructures portfolio can be browsed from ESFRI website https://ri-portfolio.esfri.eu/.
Destination & Scope
Under destination “Biodiversity and ecosystem services”, R&I in 2025 provides scientific support to the development and implementation of EU environmental legislation and of European Green Deal initiatives, in line with the new Commission priority “Sustaining our quality of life: food security, water and nature”.
This destination is based on the vision developed in the EU biodiversity strategy for 2030 and supports its implementation, pursuing the orientations of the Work Programmes 2021-2022 and 2023-2024, and notably focuses on the EU Nature Restoration Regulation and other new European Green Deal initiatives such as the proposal for an EU soil monitoring and resilience law, the proposal for an EU forest monitoring law and the EU Taxonomy for Sustainable Activities (specifically the Environmental Delegated Act) and the EU action plan: protecting and restoring marine ecosystems for sustainable and resilient fisheries. R&I activities continue to support the environmental objectives of the common agricultural policy and reflect the strong interconnections between the EU biodiversity strategy for 2030 and the European Green Deal objectives for a competitive, resilient and sustainable agri-food system, including the pollinators initiative.
R&I on biodiversity and ecosystems services, if translated into action, contribute to a clean environment for the EU and Associated Countries, including water, soil, air, health, climate adaptation and risk (including disaster risk) reduction, sustainable bioeconomy and blue economy policies.
This destination also contributes to the twin green and digital transition. Where relevant, advantage should be taken of the use of advanced digital technologies and tools such high-performance computing, Artificial Intelligence (AI) and Environmental Observation where appropriate.
This destination supports the EU leadership in the relevant international fora and develops analysis and tools to reach our international biodiversity commitments, such as those taken in the Kunming-Montreal Global Biodiversity Framework (GBF), in line with the new Commission priorities. It will in particular support the monitoring framework of the GBF. Its activities serve the objectives of the Intergovernmental Science-Policy Platform on Biodiversity and Ecosystem Services (IPBES), the Intergovernmental Panel on Climate Change (IPCC) and of the potential International/Intergovernmental Panel for Ocean Sustainability (IPOS).
Proposals for topics under this destination should set out a credible pathway contributing to “putting biodiversity on a path to recovery, and protecting and restoring ecosystems and their services” of the Strategic Plan 2025-2027, and more specifically to one or more of the following impacts:
- improved knowledge, innovations, methods, pathways and tools are available to protect healthy ecosystems and to restore degraded ones ensuring the provision of ecosystem services, including for adaptation and/or mitigation to climate change;
- the ongoing biodiversity crisis and its consequences, the benefits of ecosystem services and the need to protect and restore them are better understood. Policymakers and all relevant sectors of society are aware and well informed thereof, and fully grasp opportunities of biodiversity protection and restoration. Society is on a path of transformative change;
- farmers, foresters, and land managers test and implement biodiversity-friendly practices while safeguarding food security and the long-term sustainability of farming and forestry;
- progress towards reaching the goals and targets of the Kunming-Montréal Global Biodiversity Framework contributes to reducing the pressure on biodiversity and to ensuring sustainable development worldwide.
R&I under Destination “Biodiversity and ecosystem services” will mostly deliver under Key Strategic Orientation (KSO) 1 of Horizon Europe Strategic Plan 2025-2027: Green transition and to a lesser extent KSO 3: A more resilient, competitive, inclusive and democratic Europe.
Spending under this destination counts 100% against the target for biodiversity expenditure under Horizon Europe. In addition, most of the activities, especially in the area of ecosystem restoration, contribute to the target for climate expenditure in line with the European Climate Law, which acknowledges that the restoration of ecosystems can maintain, manage and enhance natural sinks.
The Work Programme 2025 supports additional activities of the European Biodiversity Partnership Biodiversa+, while ensuring complementarity of actions with other instruments.
Synergies are sought with:
- EU missions, in particular “A Soil Deal for Europe” and “Restoring our ocean and waters by 2030” in topics dealing with nature restoration;
- Horizon Europe partnerships: in addition to Biodiversa +, several co-funded partnerships under Cluster 6 notably Water4All, sustainable blue economy and agroecology;
- JRC activities, notably the EC Knowledge Centre for Biodiversity (KCBD) and its Science Service for Biodiversity (SSBD), the Competence Centre on Participatory and Deliberative Democracy, the European Technical Support Centre for the Global Biodiversity Framework as requested by the Convention on Biological Diversity, European regional centre for biodiversity and the Global Knowledge Support Service for Biodiversity (GKSSB).
To maximise the impacts of R&I under this destination, international cooperation is encouraged in topics as appropriate. International cooperation is sought, in particular in topics that support IPBES, the implementation of the Kunming-Montreal Global Biodiversity Framework, the Sustainable Development Goals, the Paris Agreement and related international agreements such as the Agreement on the conservation and sustainable use of marine biological diversity of areas beyond national jurisdiction (BBNJ).
Under this destination there is a substantial need for more fundamental research and therefore there is a majority of Research and Innovation Actions (RIAs).
This destination benefits from interdisciplinarity and trans-disciplinarity, including the contribution of social sciences and humanities (SSH), and takes into due account gender and other social categories and their intersections to ensure promotion of democracy and a socially just transition where relevant. Citizens and stakeholders’ engagement will be sought including with living labs. The destination is expected to contribute to the new Commission priority “Protecting our democracy, upholding our values” by engaging with civil society. Furthermore, it strives to take full advantage of the potential of nature restoration and nature-based solutions, to deliver multiple social, economic and environmental co-benefits.
Eligibility & Conditions
General conditions
1. Admissibility Conditions: Proposal page limit and layout
Applicants submitting a proposal under the blind evaluation pilot (see General Annex F) must not disclose their organisation names, acronyms, logos nor names of personnel in the proposal abstract and Part B of their first-stage application (see General Annex E).
2. Eligible Countries
described in Annex B of the Work Programme General Annexes.
A number of non-EU/non-Associated Countries that are not automatically eligible for funding have made specific provisions for making funding available for their participants in Horizon Europe projects. See the information in the Horizon Europe Programme Guide.
3. Other Eligible Conditions
described in Annex B of the Work Programme General Annexes.
4. Financial and operational capacity and exclusion
described in Annex C of the Work Programme General Annexes.
5a. Evaluation and award: Award criteria, scoring and thresholds
This topic is part of the blind evaluation pilot under which first stage proposals will be evaluated blindly.
are described in Annex D of the Work Programme General Annexes.
5b. Evaluation and award: Submission and evaluation processes
are described in Annex F of the Work Programme General Annexes and the Online Manual.
5c. Evaluation and award: Indicative timeline for evaluation and grant agreement
described in Annex F of the Work Programme General Annexes.
6. Legal and financial set-up of the grants
Beneficiaries may provide financial support to third parties. The support to third parties can only be provided in the form of grants. The maximum amount to be granted to each third party is EUR 60 000.
described in Annex G of the Work Programme General Annexes.
Specific conditions
described in the [specific topic of the Work Programme]
Application and evaluation forms and model grant agreement (MGA):
Application form templates — the application form specific to this call is available in the Submission System
Standard application form (HE RIA, IA)
Standard application form (HE RIA IA Stage 1)
Evaluation form templates — will be used with the necessary adaptations
Standard evaluation form (HE RIA, IA)
Standard evaluation form (HE RIA, IA and CSA Stage 1)
Guidance
Model Grant Agreements (MGA)
Call-specific instructions
Additional documents:
HE Main Work Programme 2025 – 1. General Introduction
HE Main Work Programme 2025 – 9. Food, Bioeconomy, Natural Resources, Agriculture and Environment
HE Main Work Programme 2025 – 14. General Annexes
HE Framework Programme 2021/695
HE Specific Programme Decision 2021/764
EU Financial Regulation 2024/2509
Rules for Legal Entity Validation, LEAR Appointment and Financial Capacity Assessment
EU Grants AGA — Annotated Model Grant Agreement
Funding & Tenders Portal Online Manual
Support & Resources
Online Manual is your guide on the procedures from proposal submission to managing your grant.
Horizon Europe Programme Guide contains the detailed guidance to the structure, budget and political priorities of Horizon Europe.
Funding & Tenders Portal FAQ – find the answers to most frequently asked questions on submission of proposals, evaluation and grant management.
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The European Charter for Researchers and the Code of Conduct for their recruitment – consult the general principles and requirements specifying the roles, responsibilities and entitlements of researchers, employers and funders of researchers.
Partner Search help you find a partner organisation for your proposal.
Latest Updates
PROPOSAL NUMBERS
Call HORIZON-CL6-2025-01-two-stage has closed on 04/09/2025.
125 proposals have been submitted.
The breakdown per topic is:
- HORIZON-CL6-2025-01-BIODIV-01-two-stage: 32
- HORIZON-CL6-2025-01-BIODIV-02-two-stage: 55
- HORIZON-CL6-2025-01-CIRCBIO-01-two-stage: 31
- HORIZON-CL6-2025-01-ZEROPOLLUTION-01-two-stage: 7
Evaluation results are expected to be communicated in December 2025.
Please note that due to a technical issue, during the first days of publication of this call, the topic page did not display the description of the corresponding destination. This problem is now solved. In addition to the information published in the topic page, you can always find a full description of the 7 destinations (Biodiversity and ecosystem services; Fair, healthy and environment-friendly food systems from primary production to consumption; Circular economy and bioeconomy sectors; Clean environment and zero pollution; Land, ocean and water for climate action; Resilient, inclusive, healthy and green rural, coastal and urban communities; Innovative governance, environmental observations and digital solutions in support of the Green Deal) that are relevant for the call in the Work Programme 2025 part for “Food, Bioeconomy, Natural Resources, Agriculture and Environment”. Please select from the work programme the destination relevant to your topic and take into account the description and expected impacts of that destination for the preparation of your proposal.